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Recombinant human LBP

Description

Characteristic

The LBP is produced from human LBP transfected CHO-cells in serum free medium. Before transfection the complete human LBP-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.

huLBP-content

0.25 mg/ml phosphate-buffered saline, pH 7.2 (determined by huLBP-ELISA with defined standard)
For reconstitution add 40 µl water/ tube

Purification

By his-tag with metal affinity purification with Talon and controlled by SDS page (purity:90-95%).
Attention: His-tag has no protease site and can’t be splitted off

Endotoxin content

Less than 0.01ng/ml (determined by LAL-ELISA-test „Chromogenix“ after heating 5' 75°C)

Special note

Up to 0.2 µg/ ml LBP mediates binding of FITC-LPS (0.5µg/ml) to CD14+CHO transfectants (FACS).

Quantity

10 µg, lyophilized

Storage

long time: -80°C. Short time storage is possible at 4°C or -20°C

Attention!

The LBP will be precipitated by rapid changes of pH or temperature.
Best working temperature is ice bath.

 

 


 

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References

Grunwald, U., X. Fan, R. S. Jack, G. Workalemahu, A. Kallis, F. Stelter, C. Schütt, 1996

Monocytes can phagozytose Gram-negative bacteria by a CD14 - dependent mechanism. J. Immunol. 157: 41

 

Bochkov, V.N. Kadl,A. Huber, J. Gruber F. et al Protective role of phospholipid oxidation products in endotoxin-induced tissue damage, Letters to Nature 2002, 419: 77-81

 

He,Y.-W. Li,H. Zhang,J. et al The extracellular matrix protein mindin is apattern-recognition molecule for microbial pathogens, Nature Immunology 2003,5:88-97

 

Heinzelmann, M., Bossart, H., Heparin binds to lipopolysaccharide (LPS)-Binding protein, facilitates the transfer of LPS to CD14, and enhances LPS-induced activation of peripheral blood monocytes, J. immunol., 2005, 174: 2280-2287

 

Hermann, C. Spreitzer, I., Schröder, N.W.J. et al Cytokine induktion by purified lipoteichoic acids from various bacterial species-Role of LBP,sCD14,CD14 and failure to induce IL-12 and subsequent IFN – release, Eur. J. Immunology 2002,32(2): 541-551

 

Jack, R.S. et al.(1995): Both membrane bound and soluble forms of CD14 bind to gram negative bacteria. Eur. J. Immunol. 25, 1436

 

 

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