Monoclonal antibody to human LBP - biG 43
Description
Category |
Mouse monoclonal antibody - Protein G purified - lyophilized |
Formulation |
biG 43 was lyophilized from a concentrated protein solution (1mg/ml) containing phosphate buffered saline, pH 7.2 Content was determined by OD280nm |
Reconstitution |
For reconstitution, add 100 µl of sterile ultra-pure distilled water to each tube. This solution can then be diluted with phosphate buffered saline (PBS) or other buffers. |
Isotype |
IgG1 |
Antigen |
LBP knock out mouse immunized with immunoaffinity purified recombinant human LBP,a 58KD glycoprotein. |
Characteristic |
Without inhibition of LPS binding to membrane bound CD14, |
Application |
ELISA, LBP inhibition studies, Western Blot |
Storage |
Long time storage at -20°C or -80°C |
Quantity |
0.1 mg without NaN3 or other stabilizations |
IgG-content | 1 mg/ml |
| Stability | The lyophilized product thought stable room temperature up to 3 weeks, but is best stored desiccated at -70oC more than 1 year, at refrigerator about 10 months. Reconstituted protein should be used immediately or stored in undiluted working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% BSA). Repeated freezing and thawing should be avoided!!! |
References
Grunwald, U., X. Fan, R. S. Jack, G. Workalemahu, A. Kallis, F. Stelter, C. Schütt, 1996 Monocytes can phagozytose Gram-negative bacteria by a CD14-dependent mechanism. J. Immunol. 157: 4119
