Monoclonal antibody to human LBP - biG 412
Description
Category |
Mouse monoclonal antibody - Protein G purified - lyophilized |
Reconstitution |
For reconstitution add 50 µl distilled water. |
Isotype |
IgG1 |
Antigen |
LBP knock out mouse immunized with immunoaffinity purified recombinant human LBP,a 58KD glycoprotein. |
Special note |
It is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (LPS) to elicit immune responses by presenting the LPS to CD14 and TLR4. |
Characteristic |
Inhibition of LPS binding to membrane bound CD14, inhibition titre 1:1000 |
Application |
ELISA, LBP inhibition studies |
Storage |
Long time storage at -20°C or -80°C |
Quantity |
100 µg in PBS without NaN3 or other stabilisation before lyophilisation |
IgG-content | 2 mg/ml (determined by mouse IgG-ELISA) |
References
Kato,A., Ogasdawara, T., Homma, T., Saito, H., Matsumoto, K. Lipopolysaccaride-Binding protein critically regulates Lipopolysaccaride-induced IFN-ß Signaling pathway in human monocytes, J. Immunol. 2004, 172: 6185-6194
Vidal,K. Labeta,O.M. Schiffrin, E.J.et al Soluble CD14 in human breast milk and its role in innate immune responses Acta odontol scand 2001,59(5): 330-334
Mansson, L.E., Kjäll,P. Pellett,S.,et al. Role of the Lipopolysaccharide-CD14 complex for the activity of Hemolysin from Uropathogenic Escherichia coli,Infection and Immunity 2007, 75(2), 997-1004
Roes, Stefanie Florian Mumm, Ulrich Seydel, and Thomas Gutsmann Localization of the Lipopolysaccharide-binding Protein in Phospholipid Membranes by Atomic Force Microscopy*1 J. Biol. Chem., Vol. 281, Issue 5, 2757-2763, February 3, 2006
