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Monoclonal antibody to human LBP - biG 412

Description

Category

Mouse monoclonal antibody - Protein G purified - lyophilized

Reconstitution

For reconstitution add 50 µl distilled water.

Isotype

IgG1

Antigen

LBP knock out mouse immunized with immunoaffinity purified recombinant human LBP,a 58KD glycoprotein.

Special note

It is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (LPS) to elicit immune responses by presenting the LPS to CD14 and TLR4.

Characteristic

Inhibition of LPS binding to membrane bound CD14, inhibition titre 1:1000
Binding titre at human LBP- ELISA: 1:50.000 Antibody directly influenced interaction of CD14-LPS-LBP, binding site is at C-terminal site of LBP.

Application

ELISA, LBP inhibition studies

Storage

Long time storage at –20°C or –80°C

Quantity

100 µg in PBS without NaN3 or other stabilisation before lyophilisation

IgG-content 2 mg/ml (determined by mouse IgG-ELISA)

 

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References

Kato,A., Ogasdawara, T., Homma, T., Saito, H., Matsumoto, K. Lipopolysaccaride-Binding protein critically regulates Lipopolysaccaride-induced IFN-ß Signaling pathway in human monocytes, J. Immunol. 2004, 172: 6185-6194

 

Vidal,K. Labeta,O.M. Schiffrin, E.J.et al Soluble CD14 in human breast milk and its role in innate immune responses Acta odontol scand 2001,59(5): 330-334

 

Mansson, L.E., Kjäll,P. Pellett,S.,et al. Role of the Lipopolysaccharide-CD14 complex for the activity of Hemolysin from Uropathogenic Escherichia coli,Infection and Immunity 2007, 75(2), 997-1004

 

Roes, Stefanie Florian Mumm, Ulrich Seydel, and Thomas Gutsmann Localization of the Lipopolysaccharide-binding Protein in Phospholipid Membranes by Atomic Force Microscopy*1 J. Biol. Chem., Vol. 281, Issue 5, 2757-2763, February 3, 2006

 

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