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Monoclonal antibody to human LBP - biG 48

Description

Category

Mouse monoclonal antibody - Protein G purified - lyophilized

Reconstitution

For reconstitution add 50 µl distilled water.

Isotype

IgG1

Antigen

LBP knock out mouse immunized with immunoaffinity purified recombinant human LBP,a 58KD glycoprotein.

Special note

It is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (LPS) to elicit immune responses by presenting the LPS to CD14 and TLR4.

Characteristic

No inhibition of LPS binding to membrane bound CD14; Binding site is not directly involved in LBP-LPS-CD14 interaction.
Binding titre at human LBP- ELISA: 1:10.000.

Application

ELISA, LBP inhibition studies, Western Blot

Storage

Long time storage at –20°C or –80°C

Quantity

100 µg in PBS without NaN3 or other stabilisation before lyophilisation

IgG-content 2 mg/ml (determined by mouse IgG-ELISA)

 

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References

Okawa,T. Asai,Y. Hashimoto, M. Uchida,H. Bacterial fimbriae activate human peripheral blood monocytes utillizing TLR2,CD14 and CD11a/CD18 and cellular receptors Eur. J. Immunology 2002,32(9): 2543-2550

 

Roes, Stefanie Florian Mumm, Ulrich Seydel, and Thomas Gutsmann Localization of the Lipopolysaccharide-binding Protein in Phospholipid Membranes by Atomic Force Microscopy*1 J. Biol. Chem., Vol. 281, Issue 5, 2757-2763, February 3, 2006

 

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