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Monoclonal antibody to human LBP - biG 42

Description

Category

Mouse monoclonal antibody - Protein G purified - lyophilized

Reconstitution

For reconstitution add 100 µl distilled water.

Isotype

IgG1

Antigen

LBP knock out mouse immunized with immunoaffinity purified recombinant human LBP,a 58KD glycoprotein.

Special note

It is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (LPS) to elicit immune responses by presenting the LPS to CD14 and TLR4.

Characteristic

Without inhibition of LPS binding to membrane bound CD14, binding titre at human LBP- ELISA: 1:10.000.
Binding site is not directly involved in LBP-LPS-CD14 interaction.

Application

ELISA, LBP inhibition studies, Western Blot, immune histochemistry

Storage

Long time storage at –20°C or –80°C

Quantity

100 µg in PBS without NaN3 or other stabilisation before lyophilisation

IgG-content 1 mg/ml PBS

 

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References

Rahmana ,Md. Mizanur,c, et al. Lipopolysaccharide-binding protein: Local expression in bovine extrahepatic tissues , Veterinary Immunology and Immunopathology 2010

 

Andrä, J., Koch, M.H.J., Barels, R., Brander, K.: Biophysical Characterization of Endotoxin inactivation by antimicrobial peptide derived from Mammalian, NK-Lysi, Antimicrobial agents Chemother. 2004, 48/5: 1593-1599

 

Ren, L. et al The expression profile of LBP, membrane bound CD14, and Toll like receptors 2 and 4 in chronic periodontitis Journal of Periodontal ResearchVol.76/11 1950, 2005

 

 

Ren,Lei , Lijian Jin, W. Keung Leung Local expression of lipopolysaccharide-binding protein in human gingival tissues Journal of Periodontal Research Vol. 39 Issue 4 Page 242 August 2004

 

M. Mueller, B. Lindner, R. Dedrick, A.B. Schromm and U. Seydel, Endotoxin: physical requirements for cell activation Journal of Endotoxin Research 2005; 11; 299

 

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