>
Home    LBP    ELISA    human LBP

Enzyme Immunoassay for quantification of human LBP

cat. no

Analyte

044

human LBP–ELISA with multispecies reactivity
useful for quantification of LBP from pork, rabbit, bovine, dog and horse

 

 


Description

The human LBP kit has been developed for the quantitative measurement of natural and recombinant human LBP in serum, plasma and culture medium.

The human LBP Kit is a solid phase sandwich Enzyme-Linked-Immuno-Sorbent Assay (ELISA). Firstly the antigen (standard or sample) will be incubated in antibody-precoated ELISA-modules. During this incubation, human LBP is captured by solid bound antibody. Unbound material present in the sample is removed by washing. Now the plate will be incubated with a POD-labelled antibody specific for human LBP (second incubation). Revelation step includes substrate solution “Ready for use”.

The enzyme reaction is stopped by the addition of stopping solution and the optical density (OD) at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the OD versus the corresponding concentrations of the known standards. The human LBP concentration of samples with unknown concentrations, which are run concurrently with the standards, can be determined from the standard curve.

 

Test components for 96 wells

1

Precoated ELISA module

1 plate

Vial 2

Detecting antibody (POD-labelled monoclonal antibody to human LBP) ”Ready for use”

1 vial

Vial 3

Human LBP-standard

1 vial

Vial 4

Reference serum

1 vial

Vial 5

PBS

2 tab.

Vial 6

Dilution Buffer

1 vial

Vial 7

Tween 20

1 vial

Vial 8

Stopping solution “Ready for use”

1 vial

Vial 9

Substrate solution “Ready for use”

1 vial

Downloads

References

Schütt, C. et al. (1999) Implications for a general role of LPS-binding proteins (CD14, LBP) in combating bacterial infections. J. Endotoxin Research 5, 75-80

 

Berner,R. Fürll,B. Selter,F Schütt,Ch. et al Elevated Levels of Lipopolysaccharide-Binding Protein and Soluble CD14 in Plasma in Neonatal Early-Onset Sepsis, Clinical and Diagnostic Laboratory Immunology 2002, 9(2) 440-445

 

  Sitemap   Print   Top
  Imprint