Enzyme Immunoassay for quantification of human LBP

The human LBP kit has been developed for the quantitative measurement of natural and recombinant human LBP in serum, plasma and culture medium.

The human LBP Kit is a solid phase sandwich Enzyme-Linked-Immuno-Sorbent Assay (ELISA). Firstly the antigen (standard or sample) will be incubated in antibody-precoated ELISA-modules. During this incubation, human LBP is captured by solid bound antibody. Unbound material present in the sample is removed by washing. Now the plate will be incubated with a POD-labelled antibody specific for human LBP (second incubation). Revelation step includes substrate solution “Ready for use”.

The enzyme reaction is stopped by the addition of stopping solution and the optical density (OD) at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the OD versus the corresponding concentrations of the known standards. The human LBP concentration of samples with unknown concentrations, which are run concurrently with the standards, can be determined from the standard curve.

• Material Safety Data Sheet - human LBP Quantification ELISA kit