Monoclonal antibody to mouse LBP - biG 33



Mouse monoclonal antibody - Protein G purified - lyophilized


For reconstitution add 100 µl distilled water to obtain 1mg/ml




LBP knock out mouse immunized with recombinant mouse LBP

Special note

LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide
(LPS) to elicit immune responses by presenting the LPS to CD14 and TLR4


Inhibits binding of LPS to membrane bound CD14,
(Inhibits LPS-binding (0.5 µg/ml) to CD14+CHO cells, (2 x 106/ml) up to 1:1000, total inhibition 1:5, and binding titre at mouse LBP- ELISA: 1:50.000, cross reacting with rat and human LBP


ELISA, LBP inhibition studies, Western Blot


Long time storage at -20°C or -80°C


100 µg in PBS without NaN3 or other stabilisation before lyophilisation

IgG-content 1 mg/ml PBS




Gutsmann,T. Müller, M. Wiese,A. Seydel,U. et al Dual Role of Lipopolysaccharide (LPS)-Binding Protein in Neutralization of LPS and Enhancement of LPS-Induced Activation of Mononuclear Cells,Infection and Immunity 2001,69(11): 6942-6950


Hamann,L. Schumann, R.R., Flad, H.-D. Brade, L.Rietschel,E.T. et al Binding of Lipopolysaccharide(LPS) to CHO cells does not correlate with LPS-induced NF-kactivation, Eur. J. Immunology 2002,30(1): 211-216


Morath, S., Geyer, A., Hartung, T. Stucture-Function Relationship of cytokine induction by lipoteichoic acid from Staphylococcus aureus, J. Experimental Medicine 193, 2001, 393-398


Mueller, M., K. Brandenburg, R. Dedrick, A.B. Schromm, U. Seydel Phospholipids inhibit lipopolysaccharide (LPS)-induced cell activation: A role for LPS-Binding protein, J. Immunol. 2005, 174: 1091-1096


Stamme,C. Müller, M. Hamann L. Gutsmann, Th. Seydel,U. et al Surfactant Protein A Inhibits Lipopolysaccharide-Induced Immune Cell Activation by Preventing


Roes, Stefanie Florian Mumm, Ulrich Seydel, and Thomas Gutsmann Localization of the Lipopolysaccharide-binding Protein in Phospholipid Membranes by Atomic Force Microscopy*1 J. Biol. Chem., Vol. 281, Issue 5, 2757-2763, February 3, 2006


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