Monoclonal antibody to human CD14 - biG 2 (RoMo-1)
Description
Category |
Mouse monoclonal antibody protein G purified lyophilized |
Reconstitution |
For reconstitution add 100 µl distilled water |
Isotype |
IgG2a |
Antigen |
Human monocytes |
Special note |
biG 2 (RoMo-1) represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin. |
Characteristic |
no significant inhibition of LPS binding to CD14, binding epitope aa 147 -152 |
Application |
Suitable for Immunostaining of CD14 positive cells (Flow cytometry), Immunopreparation, ELISA |
Storage |
Long time storage at -20°C or -80°C |
Quantity |
100 µg in PBS without NaN3 or other stabilisation before lyophilisation |
IgG-content | 1 mg/ml PBS |
References
Dziarski.R. Tapping,R.I. Tobias P.S. et al Binding of bacterial Peptidoglycan to CD14, J. Biol Chem.1998,273(15) 8680-8690
Neumeisler, B. et al. (1998): Low endotoxic potential of Legionella pneumophila lipopolysaccharide due to failure of interaction with the monocyte lipopolysaccharide receptor CD14. Infection and Immunity 66, 4151-4157
Stelter,F. Loppnow,H. Menzel,R. Grunwald,U. et al. Differential Impact of Substitution of Amino Acids 9-13 and 91-101 of Human CD 14-Dependent Activation of Cells by Lipopolysaccharide J. of Immunology 1999,163:6035-6044
Vanlandschoot, P. Van Houtte,F. Roobrouck,A. Farhoudi,A. et al. LPS-binding protein an CD14-dependent attachment of hepatitis B Surface antigen to monocytes is determined by the phospholipid moity of the particles. J of General Virology 2002, 83:2279-2289
