Recombinant human LBP
Description
Characteristic |
The LBP is produced from human LBP transfected CHO-cells in serum free medium. Before transfection the complete human LBP-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. |
huLBP-content |
0.25 mg/ml phosphate-buffered saline, pH 7.2 (determined by huLBP-ELISA with defined standard) |
Purification |
By his-tag with metal affinity purification with Talon and controlled by SDS page (purity:90-95%). |
Endotoxin content |
Less than 0.01ng/ml (determined by LAL-ELISA-test Chromogenix after heating 5' 75°C) |
Special note |
Up to 0.2 µg/ ml LBP mediates binding of FITC-LPS (0.5 µg/ml) to CD14+CHO transfectants (FACS). |
Quantity |
10 µg, lyophilized |
Storage |
long time: -80°C. Short time storage is possible at 4°C or -20°C |
Attention!
The LBP will be precipitated by rapid changes of pH or temperature.
Best working temperature is ice bath.
References
Grunwald, U., X. Fan, R. S. Jack, G. Workalemahu, A. Kallis, F. Stelter, C. Schütt, 1996
Monocytes can phagozytose Gram-negative bacteria by a CD14 - dependent mechanism. J. Immunol. 157: 41
Bochkov, V.N. Kadl,A. Huber, J. Gruber F. et al Protective role of phospholipid oxidation products in endotoxin-induced tissue damage, Letters to Nature 2002, 419: 77-81
He,Y.-W. Li,H. Zhang,J. et al The extracellular matrix protein mindin is apattern-recognition molecule for microbial pathogens, Nature Immunology 2003,5:88-97
Heinzelmann, M., Bossart, H., Heparin binds to lipopolysaccharide (LPS)-Binding protein, facilitates the transfer of LPS to CD14, and enhances LPS-induced activation of peripheral blood monocytes, J. immunol., 2005, 174: 2280-2287
Hermann, C. Spreitzer, I., Schröder, N.W.J. et al Cytokine induktion by purified lipoteichoic acids from various bacterial species-Role of LBP,sCD14,CD14 and failure to induce IL-12 and subsequent IFN gamma release, Eur. J. Immunology 2002,32(2): 541-551
Jack, R.S. et al.(1995): Both membrane bound and soluble forms of CD14 bind to gram negative bacteria. Eur. J. Immunol. 25, 1436
