Recombinant mouse CD14
Description
Characteristic |
The mCD14 is produced from mouse CD14 transfected CHO-cells in serum free medium. Before transfection the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. |
mCD14-content |
1 mg/ml phosphate-buffered saline , pH 7.2, (determined by mouse-CD14-ELISA lyophilized For reconstitution add 10µl water to obtain 1 mg/ml |
Purification |
Purified by His-tag with metal affinity chromatography Talon (Clontech) and controlled by SDS page |
Endotoxin content |
Below 0.1 ng/ml after heating 5', 75°C (LAL-test, Chromogenix) |
Special note |
The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50 kDa protein.
|
Quantity |
10 µg |
Storage |
long time storage at –80°C |
References
Grunwald, U., X. Fan, R. S. Jack, G. Workalemahu, A. Kallis, F. Stelter, C. Schütt, 1996 Monocytes can phagozytose Gram-negative bacteria by a CD14 –dependent mechanism. J. Immunol. 157: 4119
Augusto,A.. Johansson,J Girard,R.. Chaby,R et al Interaction of Pulmonary Surfactant Protein C with CD14 and Lipopolysaccharide, Infection and Immunity 2003,71(1): 61-67
Dessing,M. et al. CD14 Facilitates respiratory tract infection by streptococcus pneumoniae , Amer. J of Respiratorical and clinical Care Medicine 2007
Berbée, Jimmy F.P., Claudia P. Coomans, Marit Westerterp Johannes A. Romijn, Louis M. Havekes, Patrick C.N. Rensen, Apolipoprotein CI Enhances the Biological Response to Lipopolysaccharide via the CD14/TLR4 Pathway by LPS-binding Elements in Both Its N- and C-Terminal Helix, J. Lipid research 2010
W. Joost Wiersinga,1,2 Alex F. de Vos,1,2 Catharina W. Wieland,1,2 Masja Leendertse,1,2 Joris J. T. H. Roelofs,3 and Tom van der Poll1,2CD14 Impairs Host Defense against Gram-NegativeSepsis Caused by Burkholderia pseudomallei in Mice, J. Infekt diseases, 2008 (198) 1388-1394
