>

Recombinant mouse CD14

Description

Characteristic

The mCD14 is produced from mouse CD14 transfected CHO-cells in serum free medium. Before transfection the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.

mCD14-content

1 mg/ml phosphate-buffered saline , pH 7.2, (determined by mouse-CD14-ELISA lyophilized

For reconstitution add 10µl water to obtain 1 mg/ml

Purification

Purified by His-tag with metal affinity chromatography Talon (Clontech) and controlled by SDS page

Endotoxin content

Below 0.1 ng/ml after heating 5', 75°C (LAL-test, Chromogenix)

Special note

The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50 kDa protein.
Up to 5µg/ml mouseCD14 inhibit binding of FITC-LPS (0.5µg/ml) to mouse CD14+CHO transfectants

 

Quantity

10 µg

Storage

long time storage at –80°C


 

Back

References

Grunwald, U., X. Fan, R. S. Jack, G. Workalemahu, A. Kallis, F. Stelter, C. Schütt, 1996 Monocytes can phagozytose Gram-negative bacteria by a CD14 –dependent mechanism. J. Immunol. 157: 4119

 

Augusto,A.. Johansson,J Girard,R.. Chaby,R et al Interaction of Pulmonary Surfactant Protein C with CD14 and Lipopolysaccharide, Infection and Immunity 2003,71(1): 61-67

 

Dessing,M. et al. CD14 Facilitates respiratory tract infection by streptococcus pneumoniae , Amer. J of Respiratorical and clinical Care Medicine 2007

 

Berbée, Jimmy F.P., Claudia P. Coomans, Marit Westerterp Johannes A. Romijn, Louis

M. Havekes, Patrick C.N. Rensen, Apolipoprotein CI Enhances the Biological Response to Lipopolysaccharide via the CD14/TLR4 Pathway by LPS-binding Elements in Both Its N- and C-Terminal Helix, J. Lipid research 2010

 

W. Joost Wiersinga,1,2 Alex F. de Vos,1,2 Catharina W. Wieland,1,2 Masja Leendertse,1,2 Joris J. T. H. Roelofs,3 and Tom van der Poll1,2CD14 Impairs Host Defense against Gram-NegativeSepsis Caused by Burkholderia pseudomallei in Mice, J. Infekt diseases, 2008 (198) 1388-1394

 

  Sitemap   Print   Top
  Imprint