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Serum free supernatant of vector transfected CHO cells
(Control supernatant for Cat No 011-3a)

Description

Characteristic

CHO-cells, transfected by expression vector p-POL- DHFR lyophilized,
for reconstitution add 100µl water

 

Medium

CHO-S-SFM-II(Gibco) + antibiotics, without NaN3

Special note

The transfected CHO cells were cultivated in a mini-reactor (same procedure like Cat 011-3a) and the supernatant than harvested in the same manner as CD14 transfected CHO cells

Quantity

100 µg

Storage

long time storage at –80°C

Attention!

Product is unpurified cell culture supernatant.

 


 

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References

Schütt, C. et al. (1999): Implications for a general role of LPS - binding proteins (CD14, LBP) in combating bacterial intections. J. Endotoxin

 

Lipsker, D. et al. (2002): Heat shock proteins 70 and 60 share common receptors which are expressed on human moncyte - derived but not epidermal dendritic cells. Eur. J. Immunol. 32

 

Jack, R.S. et al.(1995): Both membrane bound and soluble forms of CD14 bind to gram negative bacteria. Eur. J. Immunol. 25, 1436

 

Uehara, A.; Sugawara, Sh.; Tamai, R.; Takada, H.; (2001): Contrasting responses of human gingival and colonic epithelial cells to lipopolysaccharides, lipoteichoic Acid and peptidoglycans in the presence of soluble CD14. Med. Microbiol. Immunol. 189: 185-192

 

Hamann,C. Alexander, C. Stamme, U. Zähringer, R.R. Schumann Acute phase concentrations of lipopolysaccaride (LPS)-Binding protein inhibit innate immune cell activation by different LPS chemotypes via different mechanisms, Infection and Immunity 2005, 193-200 Vol 73/1

 

Rivieccio M.A., G.,R. John, X. Song, H.-S. Suh, Y. Zhao, .C. Lee C.F. Brosnan The cytokine IL1ß activates IFN response factor 3 in human fetal astrocytes in culture J. Immunol. 2005, 174: 3719-26

 

Neumeisler, B. et al. (1998): Low endotoxic potential of Legionella pneumophila lipopolysaccharide due to failure of interaction with the monocyte lipopolysaccharide receptor CD14. Infection and Immunity 66, 4151-4157

 

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