Recombinant human CD14 in serum free supernatant of CHO cells

Characteristic

The hCD14 is produced from human CD14 transfected CHO-cells. Before transfection the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.

hCD14-content

1.0 mg/ml phosphate-buffered saline, pH 7.2 determined by hCD14 ELISA with defined standard. -lyophilized-

For reconstitution add 10 µl of water to each tube

Medium

CHO-S-SFM-II(Gibco) + antibiotics, without NaN₃

Special note

The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50 kDa protein.
The transfected cells were cultivated in a mini-reactor.
50 µg/ml totally inhibits binding of FITC-LPS (0.5 µg/ml) to 6 x 10⁵ CD14⁺CHO transfectants. Up to 10 µg/ml inhibits binding of LPS-FITC.

Quantity

100 µg

Storage

long time storage at -80°C