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Recombinant human CD14 in serum free supernatant of CHO cells

Description

Characteristic

The hCD14 is produced from human CD14 transfected CHO-cells. Before transfection the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.

hCD14-content

1.0 mg/ml phosphate-buffered saline, pH 7.2 determined by hCD14 ELISA with defined standard. -lyophilized-

For reconstitution add 10 µl of water to each tube

Medium

CHO-S-SFM-II(Gibco) + antibiotics, without NaN3

Special note

The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50 kDa protein.
The transfected cells were cultivated in a mini-reactor.
50µg/ml totally inhibits binding of FITC-LPS (0.5µg/ml) to 6 x 105 CD14+CHO transfectants. Up to 10µg/ml inhibits binding of LPS-FITC.

Quantity

100 µg

Storage

long time storage at –80°C

Attention!

Product is unpurified cell culture supernatant. For special tests (ex. cell culture experiments) a control (cell culture supernatant of vector transfected CHO cells (Cat 011-3b) is necessary.

 


 

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References

Le-Barillec, K. et al. (1999): Proteolysis of monocyte CD14 by human leucocyte elastase inhibits LPS mediated cell activation. J. Clin Invest 103 (1999), 1039-46

 

Mansson, L.E., Kjäll,P. Pellett,S.,et al. Role of the Lipopolysaccharide-CD14 complex for the activity of Hemolysin from Uropathogenic Escherichia coli,Infection and Immunity 2007, 75(2), 997-1004

 

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