Recombinant human CD14
Description
Characteristic |
The hCD14 is produced from human CD14 transfected CHO-cells. Before transfection the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. |
hCD14-content |
1.0 mg/ml phosphate-buffered saline, pH 7.2 determined by hCD14 ELISA with defined standard. -lyophilized- For reconstitution add 10 µl of water to each tube |
Purification |
affinity chromatography with monoclonal antibody to hCD14 biG2 and controlled by SDS page (90-95% purity) |
Endotoxin content |
Less than 0.01 ng/ml (LAL-test Chromogenix) |
Special note |
The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50 kDa protein. Up to 10 µg/ml CD14 inhibit binding of FITC-LPS (0.5 µg/ml) to 6 x 105 CD14+CHO transfectants (FACS) |
Quantity |
10 µg |
Storage |
long time storage at -80°C |
References
Schütt, C. et al. (1999): Implications for a general role of LPS - binding proteins (CD14, LBP) in combating bacterial intections. J. Endotoxin
Lipsker, D. et al. (2002): Heat shock proteins 70 and 60 share common receptors which are expressed on human moncyte - derived but not epidermal dendritic cells. Eur. J. Immunol. 32
Jack, R.S. et al.(1995): Both membrane bound and soluble forms of CD14 bind to gram negative bacteria. Eur. J. Immunol. 25, 1436
Uehara, A.; Sugawara, Sh.; Tamai, R.; Takada, H.; (2001): Contrasting responses of human gingival and colonic epithelial cells to lipopolysaccharides, lipoteichoic Acid and peptidoglycans in the presence of soluble CD14. Med. Microbiol. Immunol. 189: 185-192
Hamann,C. Alexander, C. Stamme, U. Zähringer, R.R. Schumann Acute phase concentrations of lipopolysaccaride (LPS)-Binding protein inhibit innate immune cell activation by different LPS chemotypes via different mechanisms, Infection and Immunity 2005, 193-200 Vol 73/1
Rivieccio M.A., G.,R. John, X. Song, H.-S. Suh, Y. Zhao, .C. Lee C.F. Brosnan The cytokine IL-1β activates IFN response factor 3 in human fetal astrocytes in culture J. Immunol. 2005, 174: 3719-26
Neumeisler, B. et al. (1998): Low endotoxic potential of Legionella pneumophila lipopolysaccharide due to failure of interaction with the monocyte lipopolysaccharide receptor CD14. Infection and Immunity 66, 4151-4157
