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Recombinant human CD14

Description

Characteristic

The hCD14 is produced from human CD14 transfected CHO-cells. Before transfection the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.

hCD14-content

1.0 mg/ml phosphate-buffered saline, pH 7.2 determined by hCD14 ELISA with defined standard. -lyophilized-

For reconstitution add 10 µl of water to each tube

Purification

affinity chromatography with monoclonal antibody to hCD14 biG2 and controlled by SDS page (90-95% purity)

Endotoxin content

Less than 0.01 ng/ml (LAL-test „Chromogenix)
after heating (5', 75°C)

Special note

The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50 kDa protein.

Up to 10 µg/ml CD14 inhibit binding of FITC-LPS (0.5µg/ml) to 6 x 105 CD14+CHO transfectants (FACS)

Quantity

10 µg

Storage

long time storage at –80°C


 

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References

Schütt, C. et al. (1999): Implications for a general role of LPS - binding proteins (CD14, LBP) in combating bacterial intections. J. Endotoxin

 

Lipsker, D. et al. (2002): Heat shock proteins 70 and 60 share common receptors which are expressed on human moncyte - derived but not epidermal dendritic cells. Eur. J. Immunol. 32

 

Jack, R.S. et al.(1995): Both membrane bound and soluble forms of CD14 bind to gram negative bacteria. Eur. J. Immunol. 25, 1436

 

Uehara, A.; Sugawara, Sh.; Tamai, R.; Takada, H.; (2001): Contrasting responses of human gingival and colonic epithelial cells to lipopolysaccharides, lipoteichoic Acid and peptidoglycans in the presence of soluble CD14. Med. Microbiol. Immunol. 189: 185-192

 

Hamann,C. Alexander, C. Stamme, U. Zähringer, R.R. Schumann Acute phase concentrations of lipopolysaccaride (LPS)-Binding protein inhibit innate immune cell activation by different LPS chemotypes via different mechanisms, Infection and Immunity 2005, 193-200 Vol 73/1

 

Rivieccio M.A., G.,R. John, X. Song, H.-S. Suh, Y. Zhao, .C. Lee C.F. Brosnan The cytokine IL1ß activates IFN response factor 3 in human fetal astrocytes in culture J. Immunol. 2005, 174: 3719-26

 

Neumeisler, B. et al. (1998): Low endotoxic potential of Legionella pneumophila lipopolysaccharide due to failure of interaction with the monocyte lipopolysaccharide receptor CD14. Infection and Immunity 66, 4151-4157

 

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