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Monoclonal antibody to mouse CD14 - biG 53

Description

Category

Mouse monoclonal antibody protein G purified lyophilized

Reconstitution

For reconstitution add 100 µl distilled water

Isotype

IgG2a

Antigen

CD14 knock out mouse immunized with recombinant mouse CD14

Special note

biG 53 represents an excellent marker for mouse CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophage likes cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in mouse serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin.

Characteristic

Recognize LPS binding site at monocytes. Up to 10 µg/ml biG53 inhibit binding of LPS (0.5µg/ml) to 6 x 105 CD14+CHO transfectants (FACS), Binding titre at mouse CD14 transfected CHO-cells: more than 20.000 (ELISA, Flow cytometry)

Application

Suitable for immunostaining of CD14 positive cells and inhibition studies, ELISA
Binds at LPS activated mouse monocytes as well as mouse CD14 transfected cells or recombinant mouse CD14

Storage

Long time storage at –20°C or –80°C

Quantity

100 µg in PBS without NaN3 or other stabilisation before lyophilisation

IgG-content 1 mg/ml PBS (determined by mouse IgG-ELISA)

 

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References

Senft, A. et al. Surfactant protein-D regulates soluble CD14 though Matrix Metalloproteinase-12

J. Immunol. 2005, 174, 4953-4959

 

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