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Monoclonal antibody to mouse CD14 - biG 57

Description

Category

Mouse monoclonal antibody protein G purified lyophilized

Reconstitution

For reconstitution add 100 µl distilled water

Isotype

IgG1

Antigen

CD14 knock out mouse immunized with recombinant mouse CD14

Special note

biG 57 represents an excellent marker for mouse CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in mouse serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin.

Characteristic

No inhibition of LPS binding to CD14, binding titre at mouse CD14 transfected CHO-cells: more than 20.000 (ELISA, Flow cytometry). Recognize recombinant soluble mouse CD14

Application

Suitable for ELISA

Storage

Long time storage at –20°C or –80°C

Quantity

100 µg in PBS without NaN3 or other stabilisation before lyophilisation

IgG-content 1 mg/ml PBS (determined by mouse IgG-ELISA)

 

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References

Senft, A. et al. Surfactant protein-D regulates soluble CD14 though Matrix Metalloproteinase-12

J. Immunol. 2005, 174, 4953-4959

 

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