Monoclonal antibody to human CD14 - biG 7

Category

Mouse monoclonal antibody -protein G purified lyophilized

Reconstitution

For reconstitution add 100 µl distilled water

Isotype

IgG₁

Binding titre

1:10.000

Antigen

Monocytes and immunoaffinity purified soluble human CD14

Special note

biG 7 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin.

Characteristic

No inhibition of LPS binding to CD14, binding epitope: beyond N-terminal amino acid 1-152

Application

Suitable for immunostaining of CD14 positive cells (Flow cytometry), ELISA
CD14 inhibition studies, suitable for western blot (no reducing)

Storage

Long time storage at -20°C or -80°C

Quantity

100 µg in PBS without NaN₃ or other stabilisation before lyophilisation