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Monoclonal antibody to human CD14 - biG 7

Description

Category

Mouse monoclonal antibody -protein G purified lyophilized

Reconstitution

For reconstitution add 100 µl distilled water

Isotype

IgG1

Binding titre

1:10.000

Antigen

Monocytes and immunoaffinity purified soluble human CD14

Special note

biG 7 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin.

Characteristic

No inhibition of LPS binding to CD14, binding epitope: beyond N-terminal amino acid 1-152

Application

Suitable for immunostaining of CD14 positive cells (Flow cytometry), ELISA
CD14 inhibition studies, suitable for western blot (no reducing)

Storage

Long time storage at –20°C or –80°C

Quantity

100 µg in PBS without NaN3 or other stabilisation before lyophilisation

IgG-content 1 mg/ml PBS

 

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References

Stelter,F. Loppnow,H. Menzel,R. Grunwald,U. et al. Differential Impact of Substitution of Amino Acids 9-13 and 91-101 of Human CD 14-Dependent Activation of Cells by Lipopolysaccharide J. of Immunology 1999,163:6035-6044

 

Mansson, L.E., Kjäll,P. Pellett,S.,et al. Role of the Lipopolysaccharide-CD14 complex for the activity of Hemolysin from Uropathogenic Escherichia coli,Infection and Immunity 2007, 75(2), 997-1004

 

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