Monoclonal antibody to human CD14 - biG 4
Description
Category |
Mouse monoclonal antibody protein G purified lyophilized |
Reconstitution |
For reconstitution add 100 µl distilled water |
Isotype |
IgG1 |
Binding epitope |
aa 33-44 |
Antigen |
Monocytes of human and different animals and soluble human CD14 |
Special note |
biG 4 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin. |
Characteristic |
no significant inhibition of LPS-binding to human CD14 transfected CHO cells |
Application |
Suitable for immunostaining of CD14positive cells, ELISA, CD14 inhibition studies |
Storage |
Long time storage at -20°C or -80°C |
Quantity |
100 µg in PBS without NaN3 or other stabilisation before lyophilisation |
IgG-content | 1 mg/ml PBS |
References
Dziarski.R. Tapping,R.I. Tobias P.S. et al Binding of bacterial Peptidoglycan to CD14, J. Biol Chem.1998,273(15) 8680-8690
Stelter,F. Loppnow,H. Menzel,R. Grunwald,U. et al. Differential Impact of Substitution of Amino Acids 9-13 and 91-101 of Human CD 14-Dependent Activation of Cells by Lipopolysaccharide J. of Immunology 1999,163:6035-6044
Vanlandschoot, P. Van Houtte,F. Roobrouck,A. Farhoudi,A. et al. LPS-binding protein an CD14-dependent attachment of hepatitis B Surface antigen to monocytes is determined by the phospholipid moity of the particles. J of General Virology 2002, 83:2279-2289
Weidemann, B. Schletter,J. Dziarski,R. Ulmer,A.J. et al Specific Binding of Soluble Peptidoglycan and Muramyldipeptide to Cd14 on Human Monocytes Infection and Immunity 1997,65(3) 858-864
