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Monoclonal antibody to human CD14 - biG 2 (RoMo-1)

Description

Category

Mouse monoclonal antibody protein G purified lyophilized

Reconstitution

For reconstitution add 100 µl distilled water

Isotype

IgG2a

Antigen

Human monocytes

Special note

biG 2 (RoMo-1) represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin.

Characteristic

no significant inhibition of LPS binding to CD14, binding epitope aa 147 -152

Application

Suitable for Immunostaining of CD14 positive cells (Flow cytometry), Immunopreparation, ELISA

Storage

Long time storage at –20°C or –80°C

Quantity

100 µg in PBS without NaN3 or other stabilisation before lyophilisation

IgG-content 1 mg/ml PBS

 

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References

Dziarski.R. Tapping,R.I. Tobias P.S. et al Binding of bacterial Peptidoglycan to CD14, J. Biol Chem.1998,273(15) 8680-8690

 

Neumeisler, B. et al. (1998): Low endotoxic potential of Legionella pneumophila lipopolysaccharide due to failure of interaction with the monocyte lipopolysaccharide receptor CD14. Infection and Immunity 66, 4151-4157

 

Stelter,F. Loppnow,H. Menzel,R. Grunwald,U. et al. Differential Impact of Substitution of Amino Acids 9-13 and 91-101 of Human CD 14-Dependent Activation of Cells by Lipopolysaccharide J. of Immunology 1999,163:6035-6044

 

Vanlandschoot, P. Van Houtte,F. Roobrouck,A. Farhoudi,A. et al. LPS-binding protein an CD14-dependent attachment of hepatitis B Surface antigen to monocytes is determined by the phospholipid moity of the particles. J of General Virology 2002, 83:2279-2289

 

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