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Monoclonal antibody to human and bovine monocytes (CD14) - biG 14

Description

Category

Protein G purified, lyophilized

Reconstitution

For reconstitution add 100 µl distilled water.

Isotype

IgG2a

Antigen

Monocytes of different species and immunoaffinity purified soluble human CD14

Special note

biG 14 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin.

Characteristic

1 µg/ml inhibits binding of 0.5 µg/ml LPS to CD14, binding epitope amino acid 39-44
Cross reacting with CD14 of bovine++, porc++, dog+, horse+, guinea pig+
Inhibits binding of LPS to CD14 of porc++, dog+, bovine +, dog+
Binding titre at human CD14 transfected CHO-cells: more than 10.000

Application

Suitable for Immunostaining of CD14 positive cells (Flow cytometry), ELISA, CD14 inhibition studies, Western Blot

Storage

Long time storage at –20°C or –80°C

Quantity

100 µg in PBS without NaN3 or other stabilisation before lyophilisation

IgG-content 1 mg/ml PBS

 

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References

Bache C.N.E,Entwicklung eines Tests zur Ablösung von Tierversuchen beim Nachweis von Pertussis-Toxin, Dissertation, J. Gutenberg-Universität Mainz 2009,

 

Dziarski.R. Tapping,R.I. Tobias P.S. et al Binding of bacterial Peptidoglycan to CD14, J. Biol Chem.1998,273(15) 8680-8690

 

Fan,X. Selter,F. Menzel,R. Jack,R.. et al Structures in Bacillus subtilis are recognized by CD14 in a Lipopolysaccharide-Binding Protein-Dependent Reaction, Infection and Immunity 1999,67(6): 2964-2968

 

Jacobi, A. M. Zur Bedeutung des Prolaktins für Pathogenese und krankheitsverlauf bei systemischen Lupus erythematodes (SLE), Dissertation, Humboldt-Uni Berlin A.M. Jacobi 2000

 

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