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Monoclonal antibody to human and bovine monocytes (CD14) - biG 13

Description

Category

Protein G purified, lyophilized

Reconstitution

For reconstitution add 100µl water to get concentration of 1 mg/ml in PBS

Isotype

IgG1

Antigen

Monocytes of different species and immunoaffinity purified soluble human CD14

Special note

biG 13 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most mono¬cytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin. Repeated freezing and thawing should be avoided.

Characteristic

20 µg/ml inhibits binding of 0.5 µg/ml LPS to CD14, binding epitope amino acid 9-13, 39-44, cross reacting with CD14 of cattle++, swine ++, dog+ horse+
Binding titre at human CD14 transfected CHO-cells: more than 10.000

Application

Suitable for immunostaining of CD14 positive cells (flow cytometry) ELISA, CD14 inhibition studies, Western Blot

Storage

Long time storage at –20°C or –80°C

Quantity

100 µg in PBS without NaN3 or other stabilisation before lyophilisation

IgG-content 1 mg/ml PBS

 

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References

Saalmüller, A. Characterization of swine leukocyte differentiation antigens, Immunology Today 1996, 17 (8), 352-354

 

Ibrahim Sherif, Falko Steinbach Veterinary Immunology and Immunopathology, Non-HLDA8 animal homologue section anti-leukocyte mAbs tested for reactivity with equine leukocytes 119(1-2) 15, 2007, Pages 81-91

 

Schröder, N.W.J., Morath, S. Alexander, C., Hamann,L., Hartung, T., Zähringer, U., Göbel, U.B., Weber, J.R., Schumann, R.R. Lipoteichoic Acid (TLA) of Streptococcus pnoimoniae and staphylococcus aureus activates immune cells via Toll-like receptor (TLR)-2, Lipolpolysaccharide-binding protein (LBP) and CD14 whereas TLR-4 and MD-2 are not involved J. Biol. Chem. 278 2003, 18, 15587-94

 

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