Monoclonal antibody to human and bovine monocytes - biG 10



Mouse monoclonal antibody protein G purified (lyophilized)


reconstitute with 100 µl distilled water




Human monocytes, monocytes of different species and soluble human CD14

Special note

biG 10 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin.


inhibits binding of LPS to CD14, binding epitope: amino acid 9-13, 39-44, total inhibition of LPS-binding to CD14: 100 µg/ml
Binding titre at human CD14 transfected CHO-cells: more than 10.000


Suitable for immunostaining of CD14 positive cells (Flow cytometry)

ELISA, CD14 inhibition studies, Western Blot


Long time storage at -20°C or -80°C


100 µg in PBS without NaN3 or other stabilisation before lyophilisation

IgG-content 1 mg/ml PBS




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Bruzzone,S. De Flora, A. Usai,C.Graeff,R. et al Cyclic ADP-ribose is a second messenger in the Lipopolysaccharide-stimulated proliferation of human peripheral blood mononuclear cells, Biochemical Journal 2003,375: 395-403


Haverson, K. Monoclonal Antibody Source Laboratories and Commercial Sources,International Swine CD workshop I


Morath, S., Geyer, A., Hartung, T. Stucture-Function Relationship of cytokine induction by lipoteichoic acid from Staphylococcus aureus, J. Experimental Medicine 193, 2001, 393-398


Schröder, N.W.J., Morath, S. Alexander, C., Hamann,L., Hartung, T., Zähringer, U., Göbel, U.B., Weber, J.R., Schumann, R.R. Lipoteichoic Acid (TLA) of Streptococcus pnoimoniae and staphylococcus aureus activates immune cells via Toll-like receptor (TLR)-2, Lipolpolysaccharide-binding protein (LBP) and CD14 whereas TLR-4 and MD-2 are not involved J. Biol. Chem. 278 2003, 18, 15587-94


Sing,A. Rost,D. Roggenkamp,A. et al Yersinia V-Antigen Exploits Toll-like receptor 2 and CD14 for Interleukin 10-mediated Immunosuppression J.Exp.Med.2002,196(8) 1017-1024


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