>

Monoclonal antibody to human and bovine monocytes - biG 10

Description

Category

Mouse monoclonal antibody protein G purified (lyophilized)

Reconstitution

reconstitute with 100 µl distilled water

Isotype

IgG1

Antigen

Human monocytes, monocytes of different species and soluble human CD14

Special note

biG 10 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin.

Characteristic

inhibits binding of LPS to CD14, binding epitope: amino acid 9-13, 39-44, total inhibition of LPS-binding to CD14: 100µg/ml
Binding titre at human CD14 transfected CHO-cells: more than 10.000

Application

Suitable for immunostaining of CD14 positive cells (Flow cytometry)

ELISA, CD14 inhibition studies, Western Blot

Storage

Long time storage at –20°C or –80°C

Quantity

100 µg in PBS without NaN3 or other stabilisation before lyophilisation

IgG-content 1 mg/ml PBS

 

Back

References

Saalmüller, A. Characterization of swine leukocyte differentiation antigens, Immunology Today 1996, 17 (8), 352-354

 

Bruzzone,S. De Flora, A. Usai,C.Graeff,R. et al Cyclic ADP-ribose is a second messenger in the Lipopolysaccharide-stimulated proliferation of human peripheral blood mononuclear cells, Biochemical Journal 2003,375: 395-403

 

Haverson, K. Monoclonal Antibody Source Laboratories and Commercial Sources,International Swine CD workshop I

 

Morath, S., Geyer, A., Hartung, T. Stucture-Function Relationship of cytokine induction by lipoteichoic acid from Staphylococcus aureus, J. Experimental Medicine 193, 2001, 393-398

 

Schröder, N.W.J., Morath, S. Alexander, C., Hamann,L., Hartung, T., Zähringer, U., Göbel, U.B., Weber, J.R., Schumann, R.R. Lipoteichoic Acid (TLA) of Streptococcus pnoimoniae and staphylococcus aureus activates immune cells via Toll-like receptor (TLR)-2, Lipolpolysaccharide-binding protein (LBP) and CD14 whereas TLR-4 and MD-2 are not involved J. Biol. Chem. 278 2003, 18, 15587-94

 

Sing,A. Rost,D. Roggenkamp,A. et al Yersinia V-Antigen Exploits Toll-like receptor 2 and CD14 for Interleukin 10-mediated Immunosuppression J.Exp.Med.2002,196(8) 1017-1024

 

  Sitemap   Print   Top
  Imprint