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One step ELISA for quantification of mouse CD14

cat. no

Analyte

042

mouse sCD14 - ELISA

 

 

Description

The mouse CD14 kit has been developed for the quantitative measurement of natural and recombinant mouse CD14 in serum, plasma, other body fluids and culture medium.

Principle of the test: The mouse CD14 ELISA test kit is a solid-phase enzyme-Iinked immunosorbent assay based on the sandwich principle. Samples /standards as well as POD labelled second antibody (tracer) are incubated together at ELISA modules, precoated with antibodies for mouse CD14. During this incubation, mouse CD14 is captured by solid bound antibody. If mouse CD14 is present in the sample, the tracer antibodies will bind to the captured CD14. The excess tracer is removed by washing. Next substrate solution is added to the wells. Colour will be developed proportionally to the amount of mouse LBP present in the sample. The enzyme reaction is stopped by the addition of stopping solution and the optical density (OD) at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the OD versus the corresponding concentrations of the known standards. The mouse CD14 concentration of samples with unknown concentrations, which are run with the standards, can be determined from the standard curve.

 

Test components for 96 wells

1

Precoated ELISA module

1 plate

Vial 2

Detecting antibody (POD-labelled polyclonal antibodies to mouse CD14)

1 vial

Vial 3

CD14-standard (recombinant mouse CD14, lyophilized)

1 vial

Vial 4

Reference serum (lyophilized)

1 vial

Vial 5

PBS

2 tab.

Vial 6

Dilution buffer for samples

1 vial

Vial 7

Tween 20

1 vial

Vial 8

Stopping solution “Ready for use”

1 vial

Vial 9

Substrate solution “Ready for use”

1 vial

Vial 10

Dilution buffer for detecting antibody

1 vial

Downloads


References

Dessing, M.C. CD14 facilitates invasive respiratory tract infection by streptococcus pneumoniae, Am J of Respiritory and Critical Care Medicine 2007 Vol 175. pp. 604-611

 

Gerhold K, Avagyan A, Seib C, Frei R, Steinle J, Ahrens B, Dittrich AM, Blumchen K, Lauener R, Hamelmann E. Prenatal initiation of endotoxin airway exposure prevents subsequent allergen-induced sensitization and airway inflammation in mice, Journal of Allergy and Clinical Immunology Volume 118, Issue 3, September 2006, Pages 666-673

 

Adam A. Anas, Joppe W. R. Hovius, Cornelis van 't Veer, Tom van der Poll, Alex F. de Vos,

Role of CD14 in a Mouse Model of Acute Lung Inflammation Induced by Different Lipopolysaccharide Chemotypes PLoS ONE 5(4): 2010

 

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