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CD14 - Recombinant proteins and antibodies

CD14 is the 53-kD glycophosphatidylinositol (GPI)-linked glycoprotein and functions as high affinity endotoxin (LPS) receptor on the surface of monocytes, macrophages and granulocytes as well as brain cells. The CD14-Receptor consists of 326 amino acids. CD14 has opsonin receptor funktion and binds bacterial fatty acids and peptidoglycanes. Together with TLR4 and a lymphocytic antigen (MD2) CD14 is the lipopolysaccharide (LPS)-Receptor and mediates innate immune response to bacterial lipoproteins. In endothelial cells CD14 has regulating function for cell survival. In the presence of LPS CD14-expression can be amplified by TNF-α and IL-1β (in varied tissues differently). Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids which is directly secreted or derived from protease-dependent shedding of the membrane bound molecule. Soluble CD14 (sCD14) competes with membrane bound CD14 (mCD14) for LPS binding and is able to neutralize LPS-induced responses in vitro and in vivo and mediates the LPS-induced activation of non-CD14-expressing endothelial, epithelial and smooth-muscle cells.

 

References

Schütt, C. et al. (1999): Implications for a general role of LPS binding proteins (CD14, LBP) in combating bacterial intections. J. Endotoxin Research 5, 75-80

 

Schütt, C.; Witt, S.; Grunwald, U.; Stelter, F.; Schilling, T.; Fan, X.; Marquart, B.; Bassarab, P.; Krüger, C. (1995): Epitope mapping of CD14 glycoprotein in Leukocyte typing V, ( L. Bournsell, W. Gilks, J. M. Harlan, T. Kishimoto, C. Morimoto, J. Ritz, S. F. Schossmanns, S. Shaw, R. Silverstein, T. Springer, T. F. Tedder, R. F. Todd, eds), Oxford University Press, Oxford, 785-788

 

Stelter, F.; Bernheiden, M.; Menzel, R.; Jack, R. S.; Witt, S.; Fan, X.; Pfister, M.; Schütt, C. (1997): Mutation of amino acids 39-44 of human CD14 abrogates binding of lipopolysaccharide and Escherichia coli. Eur. J. Biochem, 243, 100-109

 

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